Antibacterial Effect of Averrhoa bilimbi Extract on Lactobacillus acidophilus ATCC 4356
Abstract
Introduction: Lactobacillus acidophilus is one of the dominant bacteria that plays a significant role in the development of dental caries. This bacterium produces extracellular polysaccharides that facilitate bacterial adhesion to dental plaque. One of the approaches to preventing dental caries is the use of antibacterial agents to inhibit bacterial growth in dental plaque. Averrhoa bilimbi (cucumber tree fruit) leaves have the potential to act as antibacterial agents due to the presence of active compounds that can inhibit bacterial growth. Aim: To determine the effect of Averrhoa bilimbi (cucumber tree fruit) leaf extract on inhibiting the growth of Lactobacillus acidophilus ATCC 4356. Methods: This study was an experimental laboratory with a post-test only control group design. The concentrations of Averrhoa bilimbi leaf extract used were 6.25%, 12.5%, 25%, and 50%, and a negative control of aquadest (sterile water). Results: The analysis with One Way ANNOVA showed p-value < 0.05, indicating a significant effect of Averrhoa bilimbi leaf extract in inhibiting the growth of Lactobacillus acidophilus ATCC 4356. Conclusion: Averrhoa bilimbi (cucumber tree fruit) leaf extract has an effect on inhibiting the growth of Lactobacillus acidophilus ATCC 4356. These findings suggest the potential of Averrhoa bilimbi leaf extract as a natural antibacterial agent
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Ferdinal N, Nazir N. Phytochemical screening and fractionation of gambier ( Uncaria gambier ). 2013;1(1):23–6.
Jiaqi S, Li T, Hongtao Z, Xiaofeng L, Tianying X. Adaptive multi-UAV path planning method based on improved gray wolf algorithm. Computers and Electrical Engineering. 2022 Dec 1;104.
Fakhra N, Gusdani A, Hidayati S, Astuti IGAK, Kementerian PK, Surabaya K. Pengetahuan Ibu Tentang Karies Gigi Pada Anak Pra Sekolah. E-Indonesian Journal of Health and Medical [Internet]. 2023;3(2). Available from: http://ijohm.rcipublisher.org/index.php/ijohm
Fitriana A, Kasuma N. Gambaran Tingkat Kesehatan Gigi Anak Usia Dini Berdasarkan Indesk def-t Pada Siswa PAUD Kelurahan Jati Kota Padang. 2016.
Robati M, Yousefimanesh H, Shokuhi far MR, Bagheri S. Effect of low-level diode laser on streptococcus mutans and lactobacillus acidophilus growth: An invitro study. J Oral Biol Craniofac Res. 2022 May 1;12(3):396–400.
Elgamily H, Safy R, Makharita R. Influence of medicinal plant extracts on the growth of oral pathogens streptococcus mutans and lactobacillus acidophilus: An in-vitro study. Open Access Maced J Med Sci. 2019 Jul 30;7(14):2328–34.
Busman, Edrizal, Utami DWP. Uji Efektivitas Ekstrak Buah Anggur Hijau (Vitis Vinivera L) Terhadap Daya Hambat Laju Pertumbuhan Bakteri Streptococcus Mutans Dan Lactobacillus Acidophilus. Ensiklopedia Social Review. 2020 Oct;325.
Singh Ahirwar S, Gupta MK, Snehi SK, Dadasaheb Kalmegh S. Dental Caries and Lactobacillus: Role and Ecology in The Oral Cavity. International Journal of Pharmaceutical Sciences and Research 4818 IJPSR [Internet]. 2019;10(11):4818–29. Available from:
http://dx.doi.org/10.13040/IJPSR.0975-8232.10
Bilqis. Nadalia Malika, Erlita I, Putri DKT. Daya Hambat Ekstrak Bawang Dayak (Eleutherine palmifolia (L.) Merr.) Terhadap Pertumbuhan Bakteri Lactobacillus acidophilus. Dentin Jurnal Kedokteran Gigi. 2018 Apr;2(1):26–31.
Fitriana A, Kasuma N. Gambaran Tingkat Kesehatan Gigi Anak Usia Dini Berdasarkan Indeks Def-T Pada Siswa Paud Kelurahan Jati Kota Padang.
Lytha ), Lailia R, Rohmawati L. Formulasi Sediaan Obat Kumur Eksttrak Kulit Nanas (Ananas comosus L.) Variasi Konsentrasi Gliserin. Jurnal Inovasi Fisika Indonesia (IFI). 2023;12(3):70–6.
Saputera D, Zufira I, Yulia Budiarti L. Inhibition Activity of Belimbing Wuluh (Averrhoa bilimbi linn) Leaf Extract to Streptococcus mutans On Acrylic Plate. Dentino Jurnal Kedokteran Gigi. 2018;3(1):10–4.
Hamid EM, Thioritz E, Haryuasrani. Penggunaan Obat Kumur Larutan Lidah Buaya pada Penyembuhan Luka Pasca Scaling. Media Kesehatan Gigi [Internet]. 2023;22(1):19–24. Available from: https://ojs3.poltekkes-mks.ac.id/index.php/medgigi/index
Aseptianova A, Yuliany EH. Penyuluhan Manfaat Belimbing Wuluh (Averrhoa bilimbi Linn.) sebagai Tanaman Kesehatan di Kelurahan Kebun Bunga, Kecamatan Sukarami, Palembang. Abdihaz: Jurnal Ilmiah Pengabdian pada Masyarakat. 2020 Dec 31;2(2):52.
Fadel MN, Setyowati E, Trinovitawati Y, Sabaan W. Uji Aktivitas Antibakteri Obat Kumur Ekstrak Daun Belimbing Wuluh (Averrhoa Bilimbi L.) Terhadap Bakteri Streptococcus mutans Penyebab Karies Gigi. Jurnal Ilmu Farmasi. 2021;12(1):2685–1229.
Murniwati, Djafri D, Kurniawati B, Susi. The Effectiveness on Infusum Averrhoa bilimbi Leaves in Inhibiting the Growth of Streptococcus mutans. Cakradonya Dental Journal [Internet]. 2019;11(1):8. Available from: http://www.jurnal.unsyiah.ac.id/CDJ
Samsuar, Tri YW, Sari AM. Aktivitas Antibakteri Kombinasi Ekstrak Daun Kemangi (Ocymum x africanum L.) Dengan Daun Pepaya (Carica papaya L.) Terhadap Staphylococcus aureus. Jurnal Farmasi Lampung [Internet]. 2023 Jun;12(1):21–8. Available from: https://www.researchgate.net/publication/371989446
Restuning S, Supriyanto I, Nurnaningsih H. The Effect of A Decoction of Star Fruit Leaves (Averrhoa Bilimbi Linn) On Saliva pH. JDHT Journal of Dental Hygiene and Therapy. 2022 Oct 3;3(2):97–100.
Ehsani M, Marashi MA, Zabihi E, Issazadeh M, Khafri S. A Comparison between Antibacterial Activity of Propolis and Aloe vera on Enterococcus faecalis (an In Vitro Study). International Journal Mollecular Cell Medical Summer. 2013;02(03):110–7.
Hasim H, Arifin YY, Andrianto D, Faridah DN. Ekstrak Etanol Daun Belimbing Wuluh (Averrhoa bilimbi) sebagai Antioksidan dan Antiinflamasi. Jurnal Aplikasi Teknologi Pangan. 2019 Aug 12;8(3):86.
Panjaitan RS, Riniwasih Kadiwijati L, Seto D. Antibacterial Activity Test 70% Ethanol Extract of Starfruit Leaves (Averrhoa blimbi L.) Againts Bacteria Shigella dysenteriae. Indonesia Natural Research Pharmaceutical Journal. 2017;2(1):2502–8421.
Pramiastuti O, Sri Rejeki D. Uji Antibakteri Kombinasi Ekstrak Daun Belimbing Wuluh (Averrhoa bilimbi L) Dan Daun Kersen (Muntingia calabura L) Terhadap Staphylococcus aureus. Jurnal Ilmiah Farmasi. 2020;9(2):2020–53.
Nova Sari S, Abrar M, Daud MA. Pseudomonas aeruginosa Antibacterial Actitivities of Ethanol Extract to Robusta Coffee (Coffea canephora) against Pseudomonas aeruginosa. Jurnal Ilmiah Mahasiswa Veteriner (JIMVET). 2022;6(1):30–5.
Verdiana M, Widarta IWR, Permana IDGM. Pengaruh Jenis Pelarut Pada Ekstraksi Menggunakan Gelombang Ultrasonic Terhadap Aktivitas Antioksidan Ekstrak Kulit Buah Lemon (Citrus limon (Linn.) Burm F. Jurnal Ilmu dan Teknologi Pangan. 2018 Dec;7(4):213–22.
Sovira GDJ, Satari MH, Mariam MS. Antimicrobial properties of various solvents combinations for phytochemical fraction derived from Uncaria gambier extract against Enterococcus faecalis ATCC 29212. Padjadjaran Journal of Dentistry. 2021 Mar 31;33(1):31
DOI: https://doi.org/10.33854/jbd.v12i1.1842
DOI (pdf): https://doi.org/10.33854/jbd.v12i1.1842.g616
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