EFEK PEMBERIAN ZINK PASCA SCALING ROOT PLANNING TERHADAP KADAR MMP-8 SALIVA PADA PASIEN GINGIVITIS

haria fitri, fildzah nurul fajrin, nila kasuma, netti suharti

Abstract


Pendahuluan: Gingivitis adalah penyakit periodontal yang ringan dengan tanda gejala klinis berupa gingiva berwarna merah, membengkak dan mudah berdarah tanpa ditemukan kerusakan tulang alveolar. MMP-8 telah dikaitkan dengan diagnosis penyakit periodontal, keparahan peradangan periodontal, perkembangan dan tindak lanjut pengobatan. Zink dapat menjadi kombinasi dalam terapi periodontal pasca scaling root planning. Penulis bertujuan untuk mengetahui efek pemberian suplemetasi zink dan obat kumur mengandung zink terhadap kadar MMP-8 pasca scaling root planning pada pasien gingivitis. Metode: Subjek penelitian adalah siswa remaja umur 16-18 tahun, menderita gingivitis sedang dan gingivitis berat berdasarkan parameter pemeriksaan Gingival Index dan Bleeding on Probing. Teknik pengambilan sampel menggunakan metode Matching. Subjek dibagi menjadi tiga kelompok perlakuan. Kadar MMP-8 saliva diperiksa dengan mengguankan ELISA kit. Efek pemberian suplementasi zink dan obat kumur mengandung zink terhadap penurunan rerata kadar MMP-8 dibandingkan dengan kelompok kontrol. Hasil: Secara statistik tidak ada perbedaan yang bermakna (p>0,05), tetapi rerata kadar MMP-8 pada kelompok suplementasi zink lebih rendah dibanding kelompok obat kumur mengandung zink dan kelompok kontrol. Simpulan: Terapi kombinasi zink memberikan efek penyembuhan yang baik terhadap gingivitis pasca scaling root planning dibandingkan dengan initial terapi scaling root planning saja, tetapi tidak terdapat perbedaan efek pemberian suplementasi zink dan obat kumur zink pasca scaling root planning terhadap kadar MMP-8 saliva pada pasien gingivitis.


Keywords


gingivitis, zink, MMP-8, saliva, scaling root planning

References


Ji S, Y.S. Choi, Y. Choi. 2015. Bacterian Invasion and Persistence: Critical Events in the Pathogenesis of Periodontitis. Journal Periodontal Research. 50: 570-85.

Lang, N. P., M. A. Schatzle, H. Loe. 2009. Gingivitis as a Risk Factor in Periodontal Disease. Journal of Clinical Periodontology. 36(10): 3-8.

Diah, Widodorini, T., Nugraheni, N. 2018. Perbedaan Angka Kejadian Gingivitis antara Usia Pra-Pubertas dan Pubertas di Kota Malang. E - Prodenta Journal of Dentistry. 2(1): 108-115.

Khatri, R., Deshm ukh J., Shrivastava, R., Gupta, S., Kawadkar, A., Vinaya Kumar, K. 2016. Is Periodontitis an Independent Risk Factor for Subclinical Atherosclerosis. Singapore Dental Journal. 37:9-13. doi:10.1016/j.sdj.2016.10.004.

Ghodpage, P. S.,Gupta, M. and Kolte, R. A. 2014. Influence of Phase I Periodontal Therapyon Level of Matrix Metalloproteinase 1 and Tissue Inhibitor of Metalloproteinase 1. The Saudi Dental Journal. 26(4), pp.171-175.

Sorsa, T., Gursoy, UK., Nwhator, S., Hernandez, M., Tervahartiala, T., Leppilahti, J., Gursoy, M., Könönen, E., Emingil, G., Pussinen, PJ., Mäntylä, P. 2016. Analysis of Matrix Metalloproteinases, Especially MMP-8, in Gingival Creviclular Fluid, Mouthrinse and Saliva for Monitoring Periodontal Diseases. Periodontology 2000. 70(1):142-63. Doi: 10.1111/Prd.12101.

Schatzle, M., Faddy MJ, Cullinan MP, Et Al. 2009. The Clinical Course of Chronic Periodontitis: V. Predictive Factors in Periodontal Disease. Journal of Clinical Periodontology. 36: 365–371. [Pubmed].

Ebarsole, J.L., Dawson DR 3rd., Morford L.A., Peyyala R., Miller C.S., Gonzales OA. 2013. Periodontal Disease Immunology:’ Double Indemnity’ in Protecting the Host. Periodontal 2000. 62: 163-201 [PubMed: 23574466].

Yucel and Bage-Linberg, T., Dan T. Bage. 2013. Inflammatory Mediators in The Pathogenesis of Periodontitis. Experts Review in Molecular Medicine. 15: 1-2

Miller CS, Foley JD, Bailey AL, Et Al. 2010. Recent Developments in the Diagnosis of Saliva. Biomarkers in Medicine Journal. 4: 171-189.

Hernandez-Rios P, Hernandez M, Garrido M, Tervahartiala T, Leppilahti J, Kuula H, Heikkinen AM, Mäntylä P, Rathnayake N, Nwhator S, Sorsa T. 2016. Oral Fluid Matrix Metalloproteinase (MMP)-8 as a Diagnostic Tool in ChronicPeriodontitis.Volume2016:3Page11-18. Https://Doi.Org/10.2147/MNM.S89245.

Heikkinen AM, Sorsa T, Pitkaniemi J, Tervahartiala T, Kari K, Broms U, Koskenvuo M, Meurman JH. 2010. Smoking Affects Diagnostic Salivary Periodontal Disease Biomarker Levels in Adolescents. Journal of Periodontology. 81:1299–307.

Zirta, U. A., Gunawan, J. A., & Nurrohman, H. 2009. Peranan Matrix Metalloproteinases dalam Karies Dentin (The Role of Matrix Metalloproteinases in Dentin Caries). Jurnal PDGI. 58(2): 25–31.

Ridwan, R.D., 2012. The Role of Actinobacillus Actinomycetemcomitans Fimbrial Adhesin on MMP-8 Activity in Agressive Periodontitis Pathogenesis. Majalah Kedokteran Gigi. 45(4):181-186.

Akbari, G., M.L.V. Prabhuji, B.V. Karthikeyan, K. Raghunatha, R. Narayanan. 2015. Analysis of Matrix Metalloproteinase-8 Levels in Gingival Crevicular Fluid and Whole Mouth Fluid Among Smokers and Nonsmokers Using Enzyme-Linked Immune-Sorbent Assay and A Novel Chair-Side Test. Journal of Indian Society of Periodontology. 19(5): 525-530.

Newman, M., H. Takei, P. Klokkevold., F. Carranza. 2015. Carranza’sClinical Periodontology 12thed. Canada: Elsevier Saunders. 215 p.

Kasuma, N., F. Oenzil, And N.I. Lipoeto. 2016. Correlation Between Matrix Metalloproteinase 8 In Gingival Crevicular Fluid and Zinc Consumption. Pakistan Journal of Nutrition. 15(1): 72-75.

Nagasri, M., Madhulatha, M., Musalaiah, SV., Kumar, PA., Krishna, CH., Kumar, PM. 2015. Efficacy of Curcumin as an Adjunct to Scaling and Root Planning in Chronic Periodontitis Patients: A Clinical and Microbiological Study. Journal of Pharmacy & Bioallied Sciences. 7 (2). doi: 10.4103/0975-7406.163537.

Vyas, M., Dan S. Vyas. 2018. Assessment Of Tetracycline As An Adjunct To Scaling and Root Planing in Periodontitis Patients. International Journal of Contemporary Medicine Surgery and Radiology. 3(1): 128-128.

Tanwar, J., S. A. Hungund, K. Dodani. 2016. Nonsurgical Periodontal Therapy: A Review. Departement of Periodontics, Darshan Dental College and Hospital, Udaipur, Rajasthan. India 8(1): 39-44.

Kumar, A., S.M. Jan, Dan A.F. Shah. 2017. Evaluation of the Beneficial Clinical Effects of Scalling and Root Planning With 0,2% Chlorhexidine Over Scaling and Root Planning as a Monothreapy. Scholars Journal of Dental Science. 4(5): 220-225.

Williams,RC., Paquette,DW., Offenbacher,S., Adams,DF., Armitage, GC., Bray, K., Caton,J., Cochran,DL., Drisko,CH., Fiorellini,JP., Giannobile,WV., GrossiS., Guerrero,DM., Johnson,GK., Lamster,IB., Magnusson,I., Oringer,RJ., Persson, GR., Van Dyke, TE., Wolff, LF., Santucci,EA., Rodda,BE., Lessem, J. 2001. Treatment of Periodontitis by Local Administration of Minocycline Microspheres: a Controlled Trial. Journal of Periodontologi. 72(11):1535-44.

Reynolds, E. 2008. Effeciency and Effectiveness in Ultrasonic Scaling. American Dental Association. Los Angeles: CERP 1-7.

Uraz, A., Karaduman, B. Sila, C. 2019. ‘Sciencedirect Ozone Application as Adjunctive Therapy in Chronic Periodontitis : Clinical , Microbiological and Biochemical Aspects’. Doi: 10.1016/J.Jds.2018.06.005.

Lynch, R.J.M. 2011. Zinc In Mouth, Its Interactions With Dental Enamel and Possible Effects on Caries: A Review of The Literature. International Dental Journal. 61(3): 46-54.

Mujico, J.R., Pérez-de-Heredia F., Gómez-Martínez S, Marcos A. 2012. Malnutrition and Inflammation. In: Mahin K, Inflammation Chronic Diseases and Cancer - Cell and Molecular Biology, Immunology and Clinical Bases. 367-374.

Prasad, A. S. 2009. Zinc: Role In Immunity, Oxidative Stress and Chronic Inflammation. Current Opinion In Clinical Nutrition and Metabolic Care. 12(6): 646–652. Https://Doi.Org/10.1097/MCO.0b013e3283312956

Najeeb, S., M.S. Zafar. Z. Khurshid, S. Zohaib, and K. Almas. 2016. The Role of Nutrition in Periodontal Health: An Update. Nutrients. 8(530):1-18. Doi: 10.3390/Nu8090530.

Sadegh, A.A.M., G. Rezvaneh, E.M. Shahroo, A. Mojgan, K. Azam, R. Shahram, S.A. Reza, M. Nafiseh. 2016. Zinc Supplementation Effect on Orthodontical Tooth Movement in Rats. Dental Press Journal of Orthodontics. 21(2):45-50.

Zhang, C., X. Lu, Y. Tan, B. Li, X. Miao, L. Jin, X. Shi, X. Zhang, L. Miao, X. Li, L. Cai. 2012. Diabetes-Induced Hepatic Pathogenic Damage, Inflammation, Oxidative Stress, and Insulin Resistance was Exacerbated in Zinc Deficient Mouse Model. Plos ONE 7(12):1-7.

Chou, J., J. Hao, H. Hatoyama, B. Ben-Nissan, B. Milthorpe, M. Otsuka. 2013. The Therapeutic Effect on Bone Mineral Formation from Biomimetic Zinc Containing Tricalcium Phosphate (Zntcp) in Zinc-Deficient Osteoporotic Mice. Plos ONE 8(8):1-8. Doi: 10.1371/Journal.Pone.0071821.

Praptiwi, E. Sulistyowati, Kustiyono. 2009. Pola Makan dan Pertumbuhan Bobot Tubuh Tikus yang Diinokulasi Porphyromonas Gingivalis Sebelum dan Sesudah Terjadinya Periodontitis. Media Medika Indonesiana. 43(5): 229-233.

Bortolin, R.H., B.J.D.G.A. Abreu, M.A.G. Ururahy, K.S.C. De Souza, J.F. Bezerra, M.B. Loureiro, F.S. Da Silva, D.E.D.S. Marques, A.A.D.S. Batista, G. Oliviera, A.D. Luchessi, V.M.G.D.M. Lima, C.E.S. Miranda, M.V.L. Fook, M.D.G. Almeida, L.A. De Rezende, A.A. De Rezende. 2015. Protection Against T1DM-Induced Bone Loss by Zinc Suppleetation: Biomechanical, Histomorphometric, and Molecular Analyses In STZ-Induced Diabetic Rats. P Los ONE. 10(5): 1-18. Doi:10.1371/Journal.Pone.0125349.

nJarosz, M., Olbert, M., Wyszogrodzka, G., Mlyniec, K., and Librowski, T. 2107. Antioxidant and Anti-inflammatory Effects of Zinc, Zinc-dependent NF-kB Signaling. Inflammopharmacology. 25(1), 11-24.

Licthen, L. A., and R.J. Cousin. 2009. Mammalian Zinc Transsporter: Nutritional and Physiologic Regulation. Annual Review of Nutrition. 29: 153-176.

Lansdown, A. B., Mirastschijski, U., Stubbs, N., Scanlon, E., and Agren, M. S.2007. Zinc Wound Healing: Theoretical, Experimental, and Clinical Aspect. Wound Repair and Regeneration. 15(1): 2-16.

Balli, U., B.O. Cetinkaya, G.C. Keles, Z.P. Keles, S. Guler, M.U. Sogut, Z. Erisgin. 2016. Assessment of MMP-1, MMP-8 and TIMP-2 in Experimental Periodontitis Treated with Kaempferol. Journal of Periodontal and Implant Science. 46(2): 84-95.

Iii, D., Ebersole, J. L., Ph, D., & Miller, C. S. 2012. Salivary Biomarkers of Periodontal Disease in Renspon to Treatment. Journal of Clinical Periodontology. 38(5): 434–441. Https://Doi.Org/10.1111/J.1600-051X.2011.01706.X.

Agrawal, A, Diego, R.P, Jenifer, A, Fransisco J.V, Seth, M.C. 2010. Zinc-Binding Grups Modulate Selektif Inhibition of Mmps. Chemmedchem; 3(5): 812-820.

Konopka, Ł., Pietrzak, A., & Effect, E. 2012. Effect of Scaling and Root Planing on Interleukin-1 B , Interleukin-8 and MMP-8 Levels in Gingival Crevicular Fluid from Chronic Periodontitis Patients. Journal Periodontal Research. 47(6):681-8. Https://Doi.Org/10.1111/J.1600-0765.2012.01480.X.

O ̈zdemir A, Sayal A, Akca Eet al. 1998. The Determination of Salivaryzinc Level Following Delivery from Zinc-Containing Toothpaste. International Journal of Medical Sciences. 28: 281–283.

Tanaka M, Matsunaga K, Kadoma Y. 2000. Correlation in Inorganicion Concentration Between Saliva and Plaque Fluid. Journal of Medical Dental Sciences. 47: 55–59.

Jiyani, T. 2015. Research Article in Combination With Sodium Fluoride ( 0 . 05 %) and Zinc Chloride. Journal of Chemical and Pharmaceutical Research. 7(6): 758–764.

Mohamed, M., Sabah, A., Ibrahim, M., Hassan, A., & Mohamad, N. 2018. Antibacterial Activity of Zinc Against Streptococcus Mutans. The Saudi Dental Journal. 30 (4): 283–291. Https://Doi.Org/10.1016/J.Sdentj.2018.06.003.

Kraków, M.C. 2016. Determination of Active Matrix-Metalloproteinase 8 (Ammp-8) Levels in the Gingival Crevicular Fluid As A Diagnostic Test During Periodontal Maintenance Therapy [Disertasi]. Berlin. Vorgelegt Der Medizinischen Fakultät Charité- Universitätsmedizin. 102 hal.

Mantyla, P., Stenman, M., Kinane, D., Salo, T., Soumalainen, K., Tikanoja, S. and Sorsa,T. 2006. Monitoring Periodontal Disease Status in Smokers and Nonsmokers Using a Gingival Fluid Matrix Metalloproteinase-8 Spesifik Chair-Side Test. Journal of Periodontal Reseacrh. 41: 503-512. Doi: 10.1111/j.1600-0765.2006.00897.x.

Kinane, D. F., Darby, I.B., Said, S., Luoto, H., Sorsa, T., Tikanoja, S. and Mantyla, P. 2003. Changes in Gingival Crevicular Fluid Martix Metalloproteinase-8 Levels During Periodontal Treatment and Maintenance. Journal Periodontal Research. 38: 400-404

Marcaccini, A. M., Meschiari, C. A., Zuardi, L. R., De Sousa, T. S., Taba, M., Jr., Teofilo, J. M., Jacob-Ferreira, A. L., Tanus-Santos, J. E., Novaes, A. B., Jr. & Gerlach, R. F. 2010. Gingival Crevicular Fluid Levels of MMP-8, MMP-9, TIMP-2, and MPO Decrease After Periodontal Therapy. Journal of Clinical Periodontology. 37: 180-190. Doi:10.1111/J.1600-051X.2009.01512.X.

Tuter, G., Serdar, M., Kurtis, B., Walker, S. G., Atak, A., Toyman, U., Pinar, S. & Aykan, T. 2010. Effects of Scaling and Root Planing and Subantimicrobial Dose Doxycycline on Gingival Crevicular Fluid Levels of Matrix Metalloproteinase-8, -13 and Serum Levels of Hscrp In Patients with Chronic Periodontitis. Journal of Periodontology. 81: 1132-1139. Doi:10.1902/Jop.2010.090694.

Emingil, G., Han, B., Ozdemir, G., Tervahartiala, T., Vural, C., Atilla, G., Baylas, H. & Sorsa, T. 2012. Effect of Azithromycin, As An Adjunct to Nonsurgical Periodontal Treatment, on Microbiological Parameters and Gingival Crevicular Fluid Biomarkers in Generalized Aggressive Periodontitis. Journal Periodontal Research. 47: 729-739. Doi:10.1111/J.16000765.2012.01488.X.

Leppilahti, J. M., Sorsa, T., Kallio, M. A., Tervahartiala, T., Emingil, G., Han, B. & Mantyla, P. 2015. The Utility of Gingival Crevicular Fluid Matrix Metalloproteinase- 8 Response Patterns in Prediction of Site-Level Clinical Treatment Outcome. Journal of Periodontology. 86: 777-787. Doi:10.1902/Jop.2015.140421.

Lee, W., Aitken, S., Sodek, J and McCulloch, C. A. 1995. Evidence of a Direct Relationship Between Neutrofil Collagenase Activity and Periodontal Tissue Destruction in Vivo: Role of Active Enzyme In Human Periodontitis. Journal Periodontal Research. 30: 23-33.

Kinney, J.S., Morelli T., Braun T., 2014. Saliva Pathogen Biomarker Signature and Periodontal Disease Progression. Journal Dental Research. 90: 752-758 [PubMed 21406610].




DOI: https://doi.org/10.33854/jbd.v6i2.268

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Creative Commons License
B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah distributed under Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.