Tes Toleransi Glukosa Oral (TTGO) Pasca Pemberian Suspensi Kedelai Dibanding Suspensi Tempe pada Tikus (Rattus norvegicus Galur Wistar) Diabetik

Huriatul Masdar, Muhammad Radhiatul Hakiki, Muhammad Rafi Syahputra, Tommy Satriasumatri, Deskafiani Putri, Rizki Bunaya, Desby Juananda

Abstract


Introduction: Genistein, daidzein and glicytein are soybean’s isoflavones which have been known having anti diabetic effect. Fermentation process of soybean can lead to formation aglycan isoflavones which have higher bioavailability than unfermented-soybean. Aims: This research was aimed to evaluate the effect of soybean and tempeh (fermented-soybean) administration to oral glucose tolerance test (OGTT) in diabetic rats. Method: Twenty male Rattus norvegicus Wistar strains were used in this experiment, divided into 4 groups: standard, negative control, soybean and tempeh group. Type 2 diabetes in rats was induced by giving streptozotocin (50 mg/kgBW) and nicotinamide (110 mg/kgBW) intraperitoneally. Blood glucose were measured by using blood glucose test meter and classified into diabetes if its level above 200 mg/dL. Soybean (200 mg/kgBW/day) and tempeh (200 mg/kgBW/day) suspension were given by using gastric tube for 30 days. Results: The results showed the means of either fasting blood glucose (FBG) or 2 hours post prandial blood glucose (2hPP) of negative control were persistent above 200 mg/dL on day 15th and 30th  (p> 0,05). In soybean group there was a decrease the mean of FBG on day 15th  (p<0.05) but did not reach the normal level, and on day 30th the mean of FBG was <200 mg/dL (p<0.05) however 2hPP blood glucose was still >200 mg/dL (p<0.05). Furthermore, in tempeh group, the means of either FBG or 2hPP blood glucose reached the normal level in day 15th and 30th (p <0.05). Conclusion: the provision of tempeh was better in controlling blood sugar levels than soybean.


Keywords


diabetes, kedelai, tempe, TTGO

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DOI: https://doi.org/10.33854/heme.v3i2.634

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