Functional Evaluation of Sciatic Nerve Anastomosis Wraped by Freeze Dried Human Amniotic Membrane in Sprague Dawley Rat

Eko Perdana Putra, Pamudji Utomo, Mujaddid Idulhaq

Abstract


Background. Peripheral nerve injury (PNI) is a common medical condition. The defected nerve, if not repaired as early as possible, can cause long-term denervation and neurotrophy failure for the target organ. This leads to a series of denervation manifestations, such as muscle atrophy, loss of sensory function, etc. and ultimately, these manifestations seriously affect the patient’s sensorimotor function.1,2  Amniotic membranes have been widely used in ophthalmology and skin injury repair because of their anti-inflammatory properties. In this study, we measured therapeutic efficacy and determined if amniotic membranes could be used for sciatic nerve repair.

Material and methods. A post test only control group design has been done  in 10 healthy Sprague Dawley rats. In all rats, a unilateral right side sciatic nerve transection was performed and reanastomosed by different methods: Group I (control group): included 5 rats, the anastomosis was done by epineural microsutures using 8/0 nylon. Group II: included 5 rats, the anastomosis was done by epineural microsutures using 8/0 nylon and  then wraped by freeze dried human amniotic membrane. Functional evaluation of nerve recovery was done over 3 weeks postoperatively using walking tract analysis and calculate using Sciatic Functional Index.

Result. Functional results showed that there was no significant difference of the sciatic functional index (SFI) between group I and group II.

Conclusion. We can conclude that during 3 weeks functional evaluation, there is no significant difference between control group and  experimental group that achieved freeze dried human amniotic membrane.

Keywords: sciatic nerve injury, freeze dried human amniotic membrane, walking tract analysis, sciatic functional index.

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References


Sondell M, Lundborg G, Kanje M. Regeneration of the rat sciatic nerve into allografts made acellular through chemieal extraction. Brain Res., 795(1-2) :44-54, 1998.

Zhang Y, Zhang Gm Liu Q, Wang Y. Effect of amnion-wrapped allogenic nerve bridging on peripheral nerve injury. Int. J. Morphol., 31(3) :980-985, 2013.

de Albornoz PM, Delgado PJ, Forriol F, Maffulli N. Non-surgical therapies for peripheral nerve injury. Br Med Bull. 2011;100:73-100

Karaman M, Tuncel A, Sheidaei S, et al. Amniotic membrane covering for facial nerve repair. Neural Regeneration Research. 2013;8(11):975-982. doi:10.3969/j.issn.1673-5374.2013.11.002.

Takahiro Nakamura, Makoto Yoshitani, Helen Rigby, Nigel J. Fullwood, Wakana Ito, Tsutomu Inatomi, et al. Sterilized, freeze-dried amniotic membrane: a useful substrate for ocular surface reconstruction. Invest Ophthalmol Vis Sci. 2004 Jan;45(1):93-9.

Sarikcioglu L, Demirel BM. Walking track analysis: an assessment method for functional recovery after sciatic nerve Injury in the rat. Folia Morphol. 2008; 68:1–7

de Medinaceli L, Freed WJ, Wyatt RJ (1982) An index of the functional condition of rat sciatic nerve based on measurements made from walking tracks. Exp Neurol, 77: 634–643.

Tsai MS, Lee JL, Chang YJ, Hwang SM. Isolation of human multipotent mesenchymal stem cells from second-trimester amniotic fluid using a novel two-stage culture protocol. Hum Reprod. 2004;19(6):1450–1456.

Forbes J, Fetterolf DE. Dehydrated amniotic membrane allografts for the treatment of chronic wounds: a case series. Journal of wound care. 2012; 21:6

Roosenblum B. Evaluation of a Human Amniotic Membrane Allograft (AMNIOEXCEL®) for Treating Challenging Diabetic Lower Extremity Ulcers. Dermascience. (2014) 1-8

Li Zhi, Qin Hanjiao, Feng Zishan, Liu Wei, Zhou Ye, Yang Lifeng, et al. Human umbilical cord mesenchymal stem cell- loaded amniotic membrane for the repair of radial nerve injury. Neural Regen Res. (2013); 8(36):3441-3448

Breshah M N, Sadakah AA, Eldrieny EA, Saad KA. Functional and histological evaluation of rat sciatic nerve anastomosis using cyanoacrylate and fibrin glue. Tanta Dental Journal: 10 (2013) 67 e 74.

Dellon, A. L. and Mackinnon, S. E. (1989), Sciatic nerve regeneration in the rat. Validity of walking track assessment in the presence of chronic contractures. Microsurgery, 10: 220–225. doi: 10.1002/micr.1920100316

Hadlock TA, Koka R. Quantification of functional recovery following rat sciatic nerve transection. Exp Neurol. 2001;168(1):192-5.




DOI: https://doi.org/10.33854/heme.v2i1.259

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