Effects of Vitamin D Supplementation on Blood Glucose Levels in Streptozotocin-Induced Diabetic Rats

Rendri Bayu Hansah, Alief Dhuha, Melya Susanti, Widia Sari, Ghaniyyatul Khudri, Kurnia Maidarmi Handayani

Abstract


Diabetes mellitus (DM) is a chronic metabolic disorder characterized by persistent hyperglycemia due to impaired insulin secretion, insulin resistance, or both. Poor glycemic control in DM leads to complications such as cardiovascular disease and nephropathy. Vitamin D has been suggested to influence glucose metabolism by improving insulin secretion and sensitivity thorugh mechanisms involving pancreatic β-cells and anti-inflammatory pathways. This study evaluates the effect of vitamin D supplementation on blood glucose levels in diabetic rats. An experimental study was conducted using 25 males Sprague Dawley rats, divided into five groups: Group A (negative control), Group B (non-DM + 415 IU/kgBW vitamin D), Group C (DM without vitamin D), Group D (DM + 415 IU/kgBW vitamin D), and Group E (DM + 1100 IU/kgBW vitamin D). Diabetes was induced using streptozotocin (50 mg/kg BW), and rats with random blood glucose levels >200 mg/dL were considered diabetic. Vitamin D supplementation was given via gavage for 30 days. Serum vitamin D and random blood glucose levels were measured 30 days after supplementation. Statistical analyses included ANOVA and Pearson correlation tests. Vitamin D supplementation significantly increased serum vitamin D levels in diabetic rats, especially in Group E (DM + 1100 IU/kgBW vitamin D; (p < 0.05). Random blood glucose levels decreased significantly in diabetic groups and Group E showed the largest glucose reduction (p < 0.001). A moderate negative correlation (r = -0.47, p = 0.017) between vitamin D levels and glucose changes was observed. Vitamin D supplementation reduces blood glucose levels in diabetic rats, with higher doses showing greater efficacy. These findings highlight vitamin D’s potential as an adjunctive therapy for diabetes management. Further research is needed to explore underlying mechanisms.


Keywords


blood glucose, diabetes mellitus, vitamin D

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References


. Goyal R, Singhal M, Jialal I. Type 2 Diabetes. StatPerls 2023.

. Al-Lawati JA. Diabetes mellitus: A local and global public health emergency! Oman Med J. 2017; 32: 177–179.

. Khan MAB, Hashim MJ, King JK, Govender RD, Mustafa H, Kaabi J Al. Epidemiology of Type 2 diabetes - Global burden of disease and forecasted trends. J Epidemiol Glob Health 2020; 10: 107–111.

. Lei X, Zhou Q, Wang Y, Fu S, Li Z, Chen Q. Serum and supplemental vitamin D levels and insulin resistance in T2DM populations: a meta-analysis and systematic review. Sci Rep 2023; 13. doi:10.1038/s41598-023-39469-9.

. Szymczak-Pajor I, Śliwińska A. Analysis of association between vitamin d deficiency and insulin resistance. Nutrients. 2019; 11. doi:10.3390/nu11040794.

. Amelia R, Said FM, Yasmin F, Harun H, Tofrizal T. The anti-inflammatory activity of probiotic Dadiah to activate Sirtuin-1 in inhibiting diabetic nephropathy progression. J Diabetes Metab Disord 2023; 22: 1425–1442.

. Sakinah EN. The Effect Of Cholecalciferol On GLUT4 Proteins Translocation Of Skeletal Muscle Cells In Streptozotocin-induced Hyperglycemia Mice. .

. Sari DR, Rimbun, Yuliawati TH, Susanto J, Gunawan A, Harjanto JM. Effect of cholecalciferol on GLUT4 expression in adipocyte of diabetic rats. J ASEAN Fed Endocr Soc 2015; 30: 190–193.

. Nadimi H, Djazayery A, Javanbakht MH, Dehpour A, Ghaedi E, Derakhshanian H et al. The effect of vitamin D supplementation on serum and muscle irisin levels, and FNDC5 expression in diabetic rats. 2019www.RBMB.net.

. Krisnamurti DGB, Louisa M, Poerwaningsih EH, Tarigan TJE, Soetikno V, Wibowo H et al. Vitamin D supplementation alleviates insulin resistance in prediabetic rats by modifying IRS-1 and PPARγ/NF-κB expressions. Front Endocrinol (Lausanne) 2023; 14. doi:10.3389/fendo.2023.1089298.

. Li X, Liu Y, Zheng Y, Wang P, Zhang Y. The effect of vitamin D supplementation on glycemic control in type 2 diabetes patients: A systematic review and meta-analysis. Nutrients. 2018; 10. doi:10.3390/nu10030375.

. Bilezikian JP, Formenti AM, Adler RA, Binkley N, Bouillon R, Lazaretti-Castro M et al. Vitamin D: Dosing, levels, form, and route of administration: Does one approach fit all? Rev Endocr Metab Disord 2021; 22: 1201–1218.

. Fuentes-Barría H, Aguilera-Eguía R, Flores-Fernández C, Angarita-Davila L, Rojas-Gómez D, Alarcón-Rivera M et al. Vitamin D and Type 2 Diabetes Mellitus: Molecular Mechanisms and Clinical Implications—A Narrative Review. Int J Mol Sci 2025; 26: 2153.

. Anggraini, D., Yaswir, R., Lillah, L., & Husni, H. (2017). Correlation of Advanced Glycation End Products with Urinary Albumin Creatinin Ratio in Patients with Type 2 Diabetes Mellitus. Indonesian Journal of Clinical Pathology and Medical Laboratory, 23(2), 107-110.

. Anggraini, D., Amran, R., & Adelin, P. (2023). Deteksi Dini Hiperglikemia pada Lansia Binaan Puskesmas Guguak Kabupaten 50 Kota. Jurnal Pengabdian Masyarakat Kesehatan (JURABDIKES), 1(1), 05-08.

. Oktabelia, L., & Anggraini, D. (2022). Hubungan Kadar Glukosa Darah Puasa Dengan Troponin I Pada Pasien Infark Miokard Akut. An-Nadaa: Jurnal Kesehatan Masyarakat (e-Journal), 9(2), 215-221.

. Anggraini, D., & Zakiyah, N. J. (2024). Risk Factors of Type 2 Diabetes Mellitus in the Elderly. Nusantara Hasana Journal, 3(10), 34-40.

. Sîrbe C, Rednic S, Grama A, Pop TL. An update on the effects of vitamin D on the immune system and autoimmune diseases. Int J Mol Sci. 2022; 23. doi:10.3390/ijms23179784.

. Moharir G, Naikawadi AA, Patil J, Bhixavatimath P, Bharatha A. Effect of vitamin D on blood sugar, HbA1c and serum insulin levels in streptozotocin-induced diabetic Rats. Maedica - A Journal of Clinical Medicine 2020; 15. doi:10.26574/maedica.2020.15.3.327.

. Musazadeh V, Kavyani Z, Mirhosseini N, Dehghan P, Vajdi M. Effect of vitamin D supplementation on type 2 diabetes biomarkers: an umbrella of interventional meta-analyses. Diabetol Metab Syndr. 2023; 15. doi:10.1186/s13098-023-01010-3.

. Wu J, Atkins A, Downes M, Wei Z. Vitamin D in Diabetes: Uncovering the Sunshine Hormone’s Role in Glucose Metabolism and Beyond. Nutrients 2023; 15: 1997.

. Vargas E, Podder V, Carrillo Sepulveda M. Physiology, Glucose Transporter Type 4. StatPearls, 2023.

. da Silva Teixeira S, Harrison K, Uzodike M, Rajapakshe K, Coarfa C, He Y et al. Vitamin D actions in neurons require the PI3K pathway for both enhancing insulin signaling and rapid depolarizing effects. Journal of Steroid Biochemistry and Molecular Biology 2020; 200. doi:10.1016/j.jsbmb.2020.105690.

. Yang L, Wang H, Liu L, Xie A. The role of insulin/IGF-1/PI3K/Akt/GSK3β signaling in parkinson’s disease dementia. Front Neurosci. 2018; 12. doi:10.3389/fnins.2018.00073.

. Argano C, Mirarchi L, Amodeo S, Orlando V, Torres A, Corrao S. The role of vitamin D and its molecular bases in insulin resistance, diabetes, metabolic syndrome, and cardiovascular disease: state of the art. Int J Mol Sci. 2023; 24. doi:10.3390/ijms242015485.

. Sung CC, Liao MT, Lu KC, Wu CC. Role of vitamin D in insulin resistance. J Biomed Biotechnol. 2012; 2012. doi:10.1155/2012/634195.




DOI: https://doi.org/10.33854/heme.v7i2.1717

DOI (PDF): https://doi.org/10.33854/heme.v7i2.1717.g613

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