THE HYDROXYAPATITE OF QUAIL EGGSHELLS (CORTURNIX COTURNIX) AS REMINERALIZATION MATERIALS IN PRIMARY TEETH (IN VITRO)
Abstract
Introduction: Caries is the most common oral health problem particularly among children. Calcium deficiency due to demineralization of the enamel could be anticipated with application of remineralization materials. One of the natural alternatives to support remineralization process is quail eggshells (Coturnix coturnix) containing high calcium carbonate as hydroxyapatite precursor. Objective: This study aims to evaluate the effectiveness of quail eggshells as hydroxiapatite materials. Methods: This study was a laboratory experimental study with pretest-posttest control group design. The sample of this study was 15 mandibular primary insisivus teeth were mounted on self cure acrylic resin, and were divided into 3 groups, the treatment group used 20% quail eggshells gel (Group A), the positive control group used CPP-ACP (Group B), and the negative control used placebo gel (Group C). The applications were conducted 30 minutes for 14 days and immersed in artificial saliva. The enamel hardness was measured using Vickers Hardness Tester. Data were analyzed using Paired T-Test and Post Hoc LSD. Result: The result of Paired T-Test showed that all the groups was statistically significant (p<0,05) that leads to remineralization process. Post hoc LSD showed that the significantly difference at posttest occurred between the treatment group againts the both control groups (p<0,05). Conclusion: It can be concluded that 20% quail eggshells gel is effective as remineralization materials in primary teeth.
Keywords
Full Text:
PDFReferences
Badan Penelitian dan Pengembangan Kesehatan Departemen Kesehatan Republik Indonesia. Riset Kesehatan Dasar (RISKESDAS) Tahun 2018. 2019. 184-185,197 p.
Mukarromah A, Dwiandhono I, Imam DNA. Differences in surface roughness of enamel after whey-extract application and cpp-acp in post extracoronal-tooth bleaching. Maj Kedokt Gigi Indones. 2018;4(1):15.
Xin X, Yuan Z, Wenyuan S, Yaling L, Xuedong Z. Dental caries: principles and management. Dental caries: principles and management. Berlin: Springer; 2016. 39 p.
Rachmawati D, Kurniawati C, Hakim L, Roeswahjuni N. Efek remineralisasi casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) terhadap enamel gigi sulung. J E-Prodenta Dent. 2019;3(2):257–62.
Widyaningtyas V, Rahayu YC, Barid I. The analysis of enamel remineralization increase in pure soy milk immersion using scanning electron microscope (SEM). J Pustaka Kesehat. 2014;2(2):258–62.
Palaniswamy UK, Prashar N, Kaushik M, Lakkam SR, Arya S, Pebbeti S. A comparative evaluation of remineralizing ability of bioactive glass and amorphous calcium phosphate casein phosphopeptide on early enamel lesion. J Dent Res. 2016;13(4):297–302.
Any S, Waladiyah F. Porositas email gigi sebelum dan sesudah aplikasi pasta cangkang telur ayam negeri. J Kedokt Gigi Univ Padjadjaran. 2019;31(3):221–7.
Mohammed H, Esmaeel S, Hashem SN, Mostafa MH. Assessment of the power of the chicken eggshell powder In remineralization of induced caries like lesions in primary teeth; an in-vitro study. J Pharm Negat Results. 2023;14(03):2192–201.
Elolimy GA. In-vitro evaluation of remineralization efficiency of chicken eggshell slurry on eroded deciduous enamel. 2020;66(3):2519–28.
Agusmawanti P, Niam MH, Sasanti GE. Hardness analysis of remineralization primary teeth enamel after the application gel of duck eggshell extract (Anas platyrhynchos domesticus) with consentration 20% and 40% In Vitro. J Dent Odonto. 2022;9(2):266–72.
Fahmy H, Batubara FY. Pengaruh waktu aplikasi pasta cangkang telur bebek (Anas platyrhynchos) Terhadap kekerasan permukaan email gigi setelah aplikasi bleaching hidrogen peroksida 40%. J Kedokt Gigi Univ Baiturrahmah B-Dent. 2022;9(2):126–34.
Mawadara PA, Mozartha M, K T. Pengaruh penambahan hidroksiapatit dari cangkang telur ayam terhadap kekerasan permukaan gic. J Mater Kedokt Gigi. 2016;2(5):8–14.
Malau ND. Manufacture and characterization Of hydroxyapatite from quail eggshell using precipitation methods. Int J Progress Sci Technol. 2021;29(1):484–90.
Azis MY, Putri TR, Aprilia FR, Ayuliasari Y, Hartini OAD, Putra MR. Eksplorasi kadar kalsium (Ca) dalam limbah cangkang kulit telur bebek dan burung puyuh menggunakan titrasi dan AAS. J al-Kimiya. 2018;5(2):74–7.
Wahyudi MD, Syahrina F, Carabelly AN, Puspitasari D. Formulasi dan uji stabilitas fisik gel ekstrak batang pisang mauli (Musa acuminata). Dentin J Kedokt Gigi. 2022;6(3):161–5.
Amalina R, Monica D, Feranisa A, Syafaat FY, Sari M, Yusuf Y. Pembuatan gel hidroksiapatit cangkang kerang-simping (Amusium pleuronectes) dan pengaruhnya setelah aplikasi di lesi white-spot email gigi. Cakradonya Dent J. 2021;13(2):81–7.
Wiryani M, Sujatmiko B, Bikarindrasari R. Pengaruh lama aplikasi bahan remineralisasi casein phosphopeptide amorphous calcium phosphate fluoride (CPP-ACPF) terhadap kekerasan email. Maj Kedokt Gigi Indones. 2016;2(3):141.
Rakha SI, Awad SM, Wahba AH. Comparison of the effect of three remineralizing agents on surface microhardness and surface roughness of primary teeth enamel. Mansoura J Dent. 2020;7(27):4–7.
Hadidi F, Haghgoo R, Kameli S, Ahmadvand M. Evaluation of Remineralizing Effects of cpp-acp and nanohydroxyapatite on erosive lesions of enamel in deciduous teeth after exposure to acetaminophen syrup: an in vitro study. Open Dent J. 2022;16(1):1–6.
Dwiandhono I, Agus Imam DN, Mukaromah A. Applications of whey extract and cpp-acp in email surface towards enamel surface hardness after extracoronal bleaching. J Kesehat Gigi. 2019;6(2):93–8.
Hidayat AN, Purbaningrum DA, Hardini N. Perbedaan antara efek perendaman dalam susu sapi dan susu kedelai murni terhadap kekerasan email gigi. J e-gigi. 2021;9(2):334–9.
Asmawati. Potensi cangkang udang (Litopenaeus vannamei) sebagai bahan remineralisasi gigi. Makassar Dent J. 2018;7(1):46–9.
Najah MI, Journal I, Najah MI, Razak A, Aida N, Shukur C, et al. Characterization of Calcium Carbonate Extracted from Eggshell Waste at Various Calcination Temperature. J Int Emerg Trends Eng Res. 2020;8(10):6725–31.
Mayta-Tovalino F, Fernandez-Giusti A, Mauricio-Vilchez C, Barja-Ore J, Guerrero ME, Retamozo-Siancas Y. The abrasive and remineralising efficacy of coturnix eggshell. J Int Dent. 2022;72(6):792–6.
DOI: https://doi.org/10.33854/jbd.v12i1.1725
DOI (PDF): https://doi.org/10.33854/jbd.v12i1.1725.g626
Refbacks
- There are currently no refbacks.
Copyright (c) 2025 Ulfa Yasmin

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.