PERBANDINGAN EFEKTIVITAS DAYA HAMBAT DADIH DENGAN YOGURT TERHADAP PERTUMBUHAN BAKTERI STREPTOCOCCUS MUTAN
DOI:
https://doi.org/10.33854/JBDjbd.9Keywords:
Dadih, Yoghurt, Bacteriosin, Agar-well Diffusion Method, Streptococcus mutansAbstract
Dadih and yoghurt are functional food products that gives health benefits because it contains probiotic bacteria. Lactic acid bacteria in probiotic foods can produce antimicrobial lsubstrates, organicacids, ethanol, hydrogenperoxide, andbacteriocins. Streptococcus mutans is a gram-positive coccibacteria that became a major factor of caries. The aim of this reseacrh is to determine the effective ness differences between dadih and yoghurt to inhibit growth of Streptococcus mutans. This research is a laboratory experimental with well agar diffusion method on blood agar as a medium to see a inhibition zone by antimicrobial activity. This research used 36 well-agar which contains 50 μl of dadih and 50 μl yoghurt respectively. Result showed that mean rank of dadih’s inhibition zone is 12,75 mean while yoghurt’s inhibition zone is 24,25. Mann-Whitney test showed that p value=0,001. There was a significant inhibition effect between dadih and yoghurt on growth of Streptococcus mutans.It concluded that dadih and yoghurt have an antimicrobial effect to inhibit growth of Streptococcus mutans. Daily consumption of probiotic foods gives benefit for oral health.References
Amanah N. Identifikasi dan Karakteristik Substrat Antimikroba dari Bakteri Asam Laktat Kandidat Probiotik yang Diisolasi dari Dadih dan Yogurt. [undergraduate thesis]. Bogor: Institut Pertanian Bogor. 2011. Indonesian.
Pasaribu RP. Isolation, Characterization and Identification of Lactic Acid Bacteria DNA From Dadih Region Aia Dingin Solok. J Chem Unand. 2013 May.
PurwatiE. Buku Ajar “Teknologi Dadiah”. Padang: Universitas Andalas. 2011.
Sunarlim R.. Potensi Lactobacillus, Sp Asal dari Dadih sebagai Starter Pada Pembuatan Susu Fermentasi Khas Indonesia. Buletin Teknologi Pascapanen Pertanian. 2009: 5.
Soeharsono, Lovita Adriani, Ratu Safitri, and Osfar Sjofjan. Probiotik Basis Ilmiah, Aplikasi dan Aspek Praktis. Widya Padjajaran: Bandung. 2011.
Forssten SD, BjörklundM,and Ouwehand AC. Streptococcus mutans, Caries and Simulation Models.Nutrients. 2010 Mar 2: 2, 290-298.
Chaiya A,S. Saraya,W. Chuakul, and R. Temsiririrkkul. Screening for Dental Caries: Preventive Activities of Medicinal Plants against Streptococcus mutans. Mahidol Univ J Pharm Sci. 2013: 40(1), 9-17.
Riset Kesehatan Dasar.Badan Penelitian Dan Pengembangan Kesehatan Kementerian Kesehatan RI Tahun 2013 [cited: 2014 Oct 24]. Available from: http://www.litbang.depkes.go.id/sites/download/rkd2013/Laporan_Riskesdas2013.PDF
Nugraha AW. Streptococcusmutans Si Plak Dimana Mana.Pharm USD Yogyakarta. 2011.
Li HUANG, Qing-an XU, Chang LIU, Ming-wen FAN, and Yu-hong LI. Anticaries DNA Vaccine-Induced Secretory Immunoglobulin A Antibodies Inhibit Formation of Streptococcus mutans Biofilms in Vitro. Acta Pharm Sinica.2012 Dec 31: 34, 239-246.
Kyle P. Heim, Paula J. Crowley, Joanna R. Long, Shweta Kailasan, Robert McKenna, and L. Jeannine Brady. An Intramolecular Lock Facilitates Folding and Stabilizes The Tertiary Structure of Streptococcus mutans Adhesin P1. PNAS.2014 Sep 30: 111(44).
Domagoj Glavina , Kristina Gor, Ilija krinjari, Dubravka Negoveti Vrani, Ketij Mehuliand Karlo Ko`ul. Effect of LGG Yoghurt on Streptococcus Mutans and Lactobacillus Spp. Salivary Counts in Children. Coll Antropol. 2012: 1, 129–132.
Bowen WH, Koo H. Biology of Streptococcus mutans-Derived Glucosyltransferases: Role in Extracellular Matrix Formation of Cariogenic Biofilms. Caries Res. 2011 Feb 23: 45, 69–86.
Prashant Babaji, Kiran Keswani, Himani Lau, Mayank Lau, Nitin Sharma, and Rohit Punga. Role of Probiotics in Oral Health: A Review of The Literature. J Educ Ethics Dent. 2012 Jul: 2(2).
Utami DA.Karakterisasi Molekular Bakteri Asam Laktat (BAL) Probiotik dengan Gen 16s rRNA yang Berpotensi Menghasilkan Bakteriosin dari Fermentasi Sirsak (Annona Maricata .L) di Sumatera Barat [Tesis].Padang: Universitas Andalas. 2011. Indonesian.
BhushanJ. and Chachra S. Probiotics – Their Role in Prevention of Dental Caries.J Oral Health Comm Dent.2010 Sep: 4(3) 78-82.
Djunaedi D. Pengaruh Probiotik Pada Respon Imun.J Kedokteran Brawijaya. 2007 April: 23(1).
Pamela Hasslöf, Maria Hedberg, Svante Twetman and Christina Stecksén-Blicks. Growth Inhibition of Oral Mutans Streptococci and Candida by Commercial Probiotic Lactobacilli – An In Vitro Study. BMC Oral Health. 2010: 10(18).
Sawitri D. Pertami, Melkior Pancasiyanuar, Sefy A. Irasari, Markus B. Rahardjo, and Wasilah. Lactobacillus acidophilus Probiotic Inhibits the Growth of Candida albicans. J Dent Indonesia . 2013: 20(3), 64-67.
Houshang J, Hassan SM, Allah DK. Isolation and Identification of Lactobacilli Found in Nomads Traditional Yogurt in the City of Jahrom Using PCR Method and, the Study of Their Interactional Effects on Streptococcus mutans as Cause of Tooth Decay Using Disc and Auger Hole Methods. Adv Environ Biol. 2014 Jul 23: 8-12, 421-427.
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