PERANAN AIR PERASAN JERUK NIPIS (Citrus aurantifolia) SEBAGAI ANTIMIKROBA TERHADAP Prevotella intermedia PADA PENCEGAHAN PERIIMPLANTITIS (SECARA IN-VITRO)
DOI:
https://doi.org/10.33854/jbd.v9i2.1176Abstract
Introduction: One of the complications of using implants in patients is peri-implantitis which occurs due to the invasion of Prevotella intermedia bacteria. Therefore, we need an agent that can play a role in inhibiting the growth of these bacteria, including lime juice. Lime (Citrus aurantifolia) is an herbal plant that is often used by the community in traditional medicine because it contains antibacterial compounds. The purpose of this study was to determine whether lime juice has antibacterial activity against the growth of Prevotella intermedia by measuring the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Method: This research is a laboratory experimental study with a post-test-only control group design approach. The method used in this research is microdilution with 10 treatments. Results: The results of the antibacterial test were carried out by counting the number of bacterial colonies on the agar media, and the data were analyzed by using the One-way ANOVA statistical test. The results showed that the least number of bacterial colonies was found at a concentration of 3.125%, namely 8.75. Conclusion: Lime juice (Citrus aurantifolia) has an antibacterial effect by inhibiting the growth of Prevotella intermedia at a concentration of 3.125% and can kill the growth of Prevotella intermedia at a concentration of 6.25%.References
Newman MG, Takei HH, Caranza FA. Clinical periodontology. 11th ed. Philadelphia: WB Saunders Co. 2011; 164-9. 2. Utomo DI, Lelyati S, Masulili C. Early detection and management of peri-implant diseases deteksi dini dan penatalaksanaan penyakit peri-implan. The 3rd Periodontic Seminar. 2017; 1(1): 70-9. 3. Klinge B, Hultin M, Berglundh, T. Peri-implantitis. Dent Clinics. 2005; 49(3): 661-76. 4. Utomo DI, Lelyati S, Masulili C. Early detection and management of peri-implant diseases deteksi dini dan penatalaksanaan penyakit peri-implan. The 3rd Periodontic Seminar. 2017; 1(1): 70-9. 5. Carroll KC, Morse SA, Mietzner T, Miller S. Medical microbiology. California: Lange medical publication. 2016; 149-80. 6. Ishiguro K, Washio J, Sasaki K, Takahashi N. Real-time monitoring of the metabolic activity of periodontopathic bacteria. J Microbiol Methods. 2015; 11(5): 22-6 7. Smeets R, Henningsen A, Jung O, Heiland M, Hammächer C, Stein JM. Definition, etiology, prevention and treatment of peri-implantitis: a review. Head & Face Med. 2014; 10(1): 1-3. 8. Farhan, LS. The microbial etiology and pathogenesis of peri-implantitis. Oral Health and Dent Management. 2018; 1-11. 9. Martin B, Tadjoedin F. Mikrobiologi periodontitis kronis: kolonisasi bakteri, patogen utama, dan virus. The Third National Scientific Seminar in Periodontics. Jakarta, Indonesia. Jakarta: IPERI. 2014; 3(1): 6-7 10. Takahashi N, Ishihara K, Kimizuka R, Okuda K, Kato T. The effects of tetracycline, minocycline, doxycycline and ofloxacin on Prevotella intermedia biofilm. Oral Microbiol and Immunology. 2016; 21(6): 366-71. 11. Chismirina S, Magistra RY. Konsentrasi hambat dan bunuh minimum ekstrak daun jeruk nipis (Citrus aurantifolia) terhadap Aggregatibacter actinomycetemcomitans secara in vitro. Cakradonya Dent J. 2016; 8(1): 68-76.
Lestari RK, Amalia E, Yuwono Y. Efektivitas jeruk nipis (Citrus aurantifolia swingle) sebagai zat antiseptik pada cuci tangan. J Kedokteran dan Kesehatan. 2018; 5(2): 55-65. 13. Hakim RF, Editia A. Pengaruh air perasan jeruk nipis (Citrus aurantifolia) terhadap pertumbuhan bakteri Lactobacillus acidophilus. J Syiah Kuala Dentistry Society. 2018; 1(3): 1-5. 14. Prastiwi SS, Ferdiansyah F. Kandungan dan aktivitas farmakologi jeruk nipis (Citrus aurantifolia swing). Farmaka. 2017; 15(2): 1-8. 15. Lauma, SW. Uji efektifitas perasan air jeruk nipis (Citrus aurantifolia) terhadap pertumbuhan bakteri Staphylococcus aureus secara in vitro. Pharmacon. 2014; 4(4): 1-15.
Hashimoto Y, Okada S, Yasuda K, Kawagoe M, Kajiya M & Tsuga K. Microbial differences between active and remission peri-implantitis. Scientifict Reports. 2022;12:5284
Ito T, Mori G, Oda Y, Hirano T, Sasaki H, Honma S, et all. Clinical evaluation of periodontal pathogen levels by real-time polymerase chain reaction in peri-implantitis patients. Int J Implant Dent. 2021; 7:105. 18. Chusniah I, Muhtadi A. Aktivitas Jeruk Nipis (citrus aurantifolia) sebagai Antibakteri, Antivirus, Antifungal, Larvasida, dan Anthelmintik. Farmaka. 2017; 15(2): 9-22. 19. Rijayanti RP. Uji aktivitas antibakteri ekstrak etanol daun mangga bacang (Mangifera Foetida L.) terhadap Staphylococcus aureus secara in vitro. Jurnal Mahasiswa PSPD FK Universitas Tanjungpura. 2014; 1(1): 11-23.
Hidayah WW, Kusrini D, Fachriyah E. Isolasi, identifikasi senyawa steroid dari daun getih-getihan (rivina humilis l.) dan uji aktivitas sebagai antibakteri. Jurnal Kimia Sains dan Aplikasi. 2016; 19(1): 7-32.
Sapara TU. Efektivitas antibakteri ekstrak daun pacar air (impatiens balsamina l.) terhadap pertumbuhan porphyromonas gingivalis. Pharmacon 2016; 5(4): 10-17.
Sakka L. Identifikasi senyawa alkaloid, flavonoid, saponin, dan tanin pada jeruk nipis (citrus aurantifolia) di kabupaten bone kecamatan lamuru menggunakan metode infusa. Jurnal ilmiah kesehatan diagnosis. 2018; 12(6): 4-670
Alzoman H, Alojaym TG, Chalikkandy SN, Mehmood A, Rashed F, Divakar DD. Comparison of an Herbal-and a 0.12% Chlorhexidine-based Oral Rinse as Adjuncts to Nonsurgical Mechanical Debridement in the Management of Peri-implant Mucositis: A Randomised Controlled Trial. Oral Health Prev Dent. 2020; 18(1): 52-645.
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